One-step gRNA design and annotation function to faciliate the design and generation of genome-wide gRNA databases for a combination of parameters such as nuclease, organism, and …
Design and Preparation Guidelines for CRISPR KI HDR Templates (GenExactTM ssDNA/GenWandTM dsDNA/GenCircleTM dsDNA) This guideline aims to assist users in …
Use of CRISPR (clustered regularly interspaced short palindromic repeats) and associated Cas enzymes for genome editing has been a major technological breakthrough, making genome …
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CRISPR 101
Synthego’s free CRISPR design tool is one of the fastest and most eficient design tools available for researchers. The tool ofers easy design of synthetic sgRNAs with up to 97% editing …
Search your gene-of-interest for GN20GG motifs. If you know the protein, go for regions with important motifs, else pick the 5’ end as reading-frame deletions should mess up the whole …
To access the CRISPR designer, click on “Custom Design” in the GeneArt CRISPR Search and Design Tool. Use this tool to analyze your sequence of interest and design de novo CRISPR …
Jun 19, 2020 · NHEJ vs HDR depends on the relative expression of enzymes from either pathway in your cell. HDR will not be useful unless you provide an exogenous DNA template for the cell …
Here we review how state-of-the-art machine learning models are enhancing gRNA design for CRISPR systems, highlight strategies for interpreting model predictions, and discuss new …
Description Designs guide sequences for CRISPR/Cas9 genome editing and provides information on sequence features pertinent to guide efficiency. Sequence features include annotated off …
To accelerate CRISPR-based targeting of RNA, Guo et al. present a resource with optimized RfxCas13d guide RNAs (gRNAs) to target messenger RNAs and noncoding RNAs in six …